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Genetic manipulation of microbes 364


 
Module code MBY 364
Qualification Undergraduate
Faculty Faculty of Natural and Agricultural Sciences
Module content

Isolation of clonable DNA (genomic libraries, cDNA synthesis) cloning vectors (plasmids, bacteriophages, cosmids) plasmid incompatibility and control of copy number. Ligation of DNA fragments, modification of DNA end and different ligation strategies. Direct and indirect methods for the identification of recombinant organisms. Characterization (polymerase chain reaction, nucleic acid sequencing) and mutagenisis of cloned DNA fragments. Gene expression in Gram negative (E.coli) Gram positive (B.subtilis) and yeast cells (S.cerevisea). Use of Agrobacterium and baculoviruses for gene expression in plant and insect cells respectively. Applications in protein engineering, diagnostics and synthesis of useful products.
Module credits 18.00
NQF Level 07
Prerequisites MBY 251 and GTS 251
Contact time 1 practical per week, 2 lectures per week
Language of tuition Module is presented in English
Department Biochemistry, Genetics and Microbiology
Period of presentation Semester 2
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